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Protein

mRNA-capping enzyme regulatory subunit

Gene

VACWR117

Organism
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulatory subunit of the mRNA cap enzyme which stabilizes the catalytic subunit and enhances its methyltransferase activity through an allosteric mechanism. Heterodimeric mRNA capping enzyme catalyzes the linkage of a N7-methyl-guanosine moiety to the first transcribed nucleotide (cap 0 structure), whereas the polymerase associated VP39 is responsible for a second methylation at the 2'-O position of the ribose (cap 1 structure).
The heterodimeric enzyme is also involved in early viral gene transcription termination and intermediate viral gene transcription initiation. Early gene transcription termination requires the termination factor VTF, the DNA-dependent ATPase NPH-I and the Rap94 subunit of the viral RNA polymerase, as well as the presence of a specific termination motif. Binds, together with RAP94, to the termination motif 5'-UUUUUNU-3' in the nascent early mRNA.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processmRNA capping, mRNA processing, Transcription, Transcription regulation, Transcription termination

Names & Taxonomyi

Protein namesi
Recommended name:
mRNA-capping enzyme regulatory subunit
Alternative name(s):
Virus termination factor small subunit
Short name:
VTF small subunit
mRNA-capping enzyme 33 kDa subunit
mRNA-capping enzyme D12 subunit
mRNA-capping enzyme small subunit
Gene namesi
Ordered Locus Names:VACWR117
ORF Names:D12L
OrganismiVaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Taxonomic identifieri10254 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
Virus hostiBos taurus (Bovine) [TaxID: 9913]
Proteomesi
  • UP000000344 Componenti: Genome

Subcellular locationi

  • Virion Curated
  • Note: All the enzymes and other proteins required to synthesize early mRNAs are packaged within the virion core along with the DNA genome.

GO - Cellular componenti

  • virion Source: UniProtKB-SubCell

Keywords - Cellular componenti

Virion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi23P → S in ts9383; conditional lethal mutant defective in the ability to convert the replicated form of the viral telomere to hairpin termini. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002101371 – 287mRNA-capping enzyme regulatory subunitAdd BLAST287

Interactioni

Subunit structurei

Heterodimer of a catalytic and a regulatory subunit. Intrinsic methyltransferase activity of the catalytic subunit is weak and needs to be stimulated 30- to 50-fold by the regulatory subunit, which is itself catalytically inert.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
VACWR106P042983EBI-16095776,EBI-16095746

Protein-protein interaction databases

DIPiDIP-60665N
IntActiP04318, 1 interactor

Structurei

Secondary structure

1287
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP04318
SMRiP04318
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP04318

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

OrthoDBiVOG090000HH

Family and domain databases

InterProiView protein in InterPro
IPR005009 Poxvirus_mRNA-cap_ssu
PfamiView protein in Pfam
PF03341 Pox_mRNA-cap, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD009934 Pox_mRNA-cap, 1 hit

Sequencei

Sequence statusi: Complete.

P04318-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDEIVKNIRE GTHVLLPFYE TLPELNLSLG KSPLPSLEYG ANYFLQISRV
60 70 80 90 100
NDLNRMPTDM LKLFTHDIML PESDLDKVYE ILKINSVKYY GRSTKADAVV
110 120 130 140 150
ADLSARNKLF KRERDAIKSN NHLTENNLYI SDYKMLTFDV FRPLFDFVNE
160 170 180 190 200
KYCIIKLPTL FGRGVIDTMR IYCSLFKNVR LLKCVSDSWL KDSAIMVASD
210 220 230 240 250
VCKKNLDLFM SHVKSVTKSS SWKDVNSVQF SILNNPVDTE FINKFLEFSN
260 270 280
RVYEALYYVH SLLYSSMTSD SKSIENKHQR RLVKLLL
Length:287
Mass (Da):33,352
Last modified:March 20, 1987 - v1
Checksum:iEA9CE30A7661A7ED
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti134K → N in CAA27369 (PubMed:3008103).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15058 Genomic DNA Translation: AAA48270.1
X03729 Genomic DNA Translation: CAA27369.1
AY243312 Genomic DNA Translation: AAO89396.1
PIRiA03892 QQVZ22
RefSeqiYP_232999.1, NC_006998.1

Genome annotation databases

GeneIDi3707515
KEGGivg:3707515

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M15058 Genomic DNA Translation: AAA48270.1
X03729 Genomic DNA Translation: CAA27369.1
AY243312 Genomic DNA Translation: AAO89396.1
PIRiA03892 QQVZ22
RefSeqiYP_232999.1, NC_006998.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2VDWX-ray2.70B/D/F/H1-287[»]
4CKBX-ray2.80B/E1-287[»]
4CKCX-ray2.90B/E1-287[»]
4CKEX-ray2.90B/E1-287[»]
ProteinModelPortaliP04318
SMRiP04318
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60665N
IntActiP04318, 1 interactor

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi3707515
KEGGivg:3707515

Phylogenomic databases

OrthoDBiVOG090000HH

Miscellaneous databases

EvolutionaryTraceiP04318

Family and domain databases

InterProiView protein in InterPro
IPR005009 Poxvirus_mRNA-cap_ssu
PfamiView protein in Pfam
PF03341 Pox_mRNA-cap, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD009934 Pox_mRNA-cap, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMCES_VACCW
AccessioniPrimary (citable) accession number: P04318
Secondary accession number(s): Q76ZR5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 1987
Last sequence update: March 20, 1987
Last modified: October 25, 2017
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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