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Protein

DNA-directed RNA polymerase 22 kDa subunit

Gene

RPO22

Organism
Vaccinia virus (strain Copenhagen) (VACV)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Part of the DNA-dependent RNA polymerase which catalyzes the transcription of viral DNA into RNA using the four ribonucleoside triphosphates as substrates. Responsible for the transcription of early, intermediate and late genes. DNA-dependent RNA polymerase associates with the early transcription factor (ETF), itself composed of D6 and A7, thereby allowing the early genes transcription. Late transcription, and probably also intermediate transcription, require newly synthesized RNA polymerase.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processTranscription

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase 22 kDa subunit (EC:2.7.7.6)
Gene namesi
Name:RPO22
ORF Names:J4R
OrganismiVaccinia virus (strain Copenhagen) (VACV)
Taxonomic identifieri10249 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000008269 Componenti: Genome

Subcellular locationi

  • Virion Curated
  • Note: All the enzymes and other proteins required to synthesize early mRNAs are packaged within the virion core along with the DNA genome. This is necessary because viral early mRNAs are synthesized within minutes after virus entry into the cell and are extruded through pores in the core particle.

GO - Cellular componenti

  • virion Source: UniProtKB-SubCell

Keywords - Cellular componenti

DNA-directed RNA polymerase, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000991391 – 185DNA-directed RNA polymerase 22 kDa subunitAdd BLAST185

Interactioni

Subunit structurei

The DNA-dependent RNA polymerase used for intermediate and late genes expression consists of eight subunits 147 kDa, 133 kDa, 35 kDa, 30 kDa, 22 kDa, 19 kDa, 18 kDa and 7 kDa totalling more than 500 kDa in mass. The same holoenzyme, with the addition of the transcription-specificity factor RAP94, is used for early gene expression (By similarity).By similarity

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

OrthoDBiVOG090000TJ

Family and domain databases

InterProiView protein in InterPro
IPR007937 RNA_Pol_22kDa_poxvir
PfamiView protein in Pfam
PF05273 Pox_RNA_Pol_22, 1 hit
PIRSFiPIRSF000744 RPO22, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD011187 RNA_Pol_22kDa_poxvir, 1 hit

Sequencei

Sequence statusi: Complete.

P68608-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNQYNVKYLA KILCLKTEIA RDPYAVINRN VLLRYTTDIE YNDLVTLITV
60 70 80 90 100
RHKIDSMKTV FQVFNESSIN YTPVDDDYGE PIIITSYLQK GHNKFPVNFL
110 120 130 140 150
YIDVVISDLF PSFVRLDTTE TNIVNSVLQT GDGKKTLRLP KMLETEIVVK
160 170 180
ILYRPNIPLK IVRFFRNNMV TGVEIADRSV ISVAD
Length:185
Mass (Da):21,342
Last modified:April 1, 1988 - v1
Checksum:iE4A289D674B3B9E0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35027 Genomic DNA Translation: AAA48084.1
PIRiA25734 RNVZ22

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35027 Genomic DNA Translation: AAA48084.1
PIRiA25734 RNVZ22

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

OrthoDBiVOG090000TJ

Family and domain databases

InterProiView protein in InterPro
IPR007937 RNA_Pol_22kDa_poxvir
PfamiView protein in Pfam
PF05273 Pox_RNA_Pol_22, 1 hit
PIRSFiPIRSF000744 RPO22, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD011187 RNA_Pol_22kDa_poxvir, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRP22_VACCC
AccessioniPrimary (citable) accession number: P68608
Secondary accession number(s): P07391
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: March 15, 2017
This is version 31 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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